This repository contains supplementary analytical datasets supporting the manuscript entitled “Multimodal Phytochemical and Micronutrient Profiling of Onosma onur-koyuncui Sezer Seeds: Metabolomic Insights and Enzyme Inhibitory Potential”. The deposited materials include representative UHPLC-HRMS/MS chromatograms, extracted ion chromatograms (EICs), MS/MS spectra of identified phenolic compounds, calibration curves, regression equations, coefficients of determination (R²), and supplementary analytical figures related to the phytochemical investigation of Onosma onur-koyuncui Sezer seeds.