A fluidic cartridge includes a sample chamber having an open top end and a sample exit port and a lysis chamber within the sample chamber. The lysis chamber is defined by an inner wall of the sample chamber, a first porous membrane affixed within the sample chamber, the first porous membrane overlapping the open top end, and a second porous membrane spaced apart from the first porous membrane affixed within the sample chamber, the second porous membrane overlapping the sample exit port. At least one magnetic element and a plurality of non-magnetic beads are contained within the lysis chamber, and the pores of the first and the second porous membranes are sized to retain the magnetic element and the non-magnetic beads within the lysis chamber.