A batch culture device includes a reaction vessel capable of holding a culture solution of a photosynthetic microorganism, a shaft extending in a central portion of an inside of the reaction vessel along a side surface of the inside of the reaction vessel, and light sources disposed in the inside of the reaction vessel and including a first light source and a second light source. An angle θ1 between a first straight line and a second straight line in a plane perpendicular to a z direction in which the shaft extends satisfies a condition 90°<θ1≤180°, where the first straight line connects a center of the first light source and a center of the shaft when seen from the z direction, and the second straight line connects a center of the second light source and the center of the shaft when seen from the z direction.