A measurement device that uses a measurement chip having a reaction region for detecting a test substance and measures a reaction of the test substance in the reaction region by using fluorescence, the measurement device including a measurement unit that irradiates the reaction region with excitation light and detects the fluorescence emitted from the reaction region, a mounting part to which the measurement chip is attachably and detachably mounted, a check chip for checking the measurement unit, the check chip being attachably and detachably mounted to the mounting part, and a storage part that stores the check chip and is provided at a location different from the mounting part.