User interface (UI) tiles of conference participants are arranged in a UI of conferencing software according to relative locations of those conference participants within a conference room. Positional information and video data are obtained from one or more video capture devices located within a conference room. Relative locations of conference participants within the conference room are determined based on the positional information, such as by defining coordinates for the conference participants within a coordinate system based on the positional information and the video data and determining the relative locations based on the coordinates. Output configured to cause a client application to arrange UI tiles associated with the conference participants is generated according to the relative locations. The output is then transmitted to one or more client devices to cause a UI of conferencing software at each of those client devices to display the UI tiles in the specified arrangement.