Abstract
The present invention provides, among other things, a method of making circular RNA by annealing a DNA splint having regions of homology to the 5′ and 3′ ends of linear RNA with an RNA ligase and subsequently removing the DNA splint. Also provided is a method of joining two linear RNA molecules by ligation using a DNA splint having regions of homology to the 3′ end of a first linear RNA and the 5′ end of a second linear RNA with a RNA ligase, and subsequently removing the DNA splints. The present invention provides a method of making circular RNA by joining two linear RNA molecules with an RNA ligase and two DNA splints, each comprising regions of homology to the 5′ end.
Full Text
What is claimed is:
The present invention provides, among other things, a method of making circular RNA by annealing a DNA splint having regions of homology to the 5′ and 3′ ends of linear RNA with an RNA ligase and subsequently removing the DNA splint. Also provided is a method of joining two linear RNA molecules by ligation using a DNA splint having regions of homology to the 3′ end of a first linear RNA and the 5′ end of a second linear RNA with a RNA ligase, and subsequently removing the DNA splints. The present invention provides a method of making circular RNA by joining two linear RNA molecules with an RNA ligase and two DNA splints, each comprising regions of homology to the 5′ end.
Timeline
Filed
03/27/2026Published
07/16/2026Granted
Not AvailableIPC Codes(7)
C12N 15/66:General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12N 9/00:Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K 8/66, A61Q 11/00; medicinal preparations containing enzymes or proenzymes A61K 38/43; enzyme containing detergent compositions C11D); Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 9/12:transferring phosphorus containing groups, e.g. kinases (2.7)