The present disclosure relates to methods for producing a genome modified non-human embryo by inducing modifications at target endogenous nucleic acid sequences. For example, methods disclosed herein may comprise providing a Cas9/sgRNA complex, comprising a Cas9 protein and a single-guide RNA (sgRNA) in a cell-free buffer. Methods may further comprise introducing the Cas9/sgRNA complex into a non-human embryo, wherein the Cas9/sgRNA complex induces a modification at a target endogenous nucleic acid of the non-human embryo to provide for a genome modified embryo. Methods may further comprise transferring the genome modified embryo into a foster mother and allowing the foster mother to produce a F0 animal having the modification at the target endogenous nucleic acid.