Provided is a method for producing a recombinant adeno-associated virus vector without using adenovirus as a helper virus. The method includes using a nucleic acid sequence encoding adenovirus L4-22K protein and/or a nucleic acid sequence encoding adenovirus L4-33K protein as a helper gene sequence for the production of the recombinant adeno-associated virus vector. A recombinant adeno-associated virus vector packaging/production cell is further provided for producing a recombinant adeno-associated virus vector without using adenovirus as a helper virus. The packaging/production cell includes adenovirus L4-22K protein or a nucleic acid encoding adenovirus L4-22K protein, and/or adenovirus L4-33K protein or a nucleic acid encoding adenovirus L4-33K protein.