/Kits And Methods For Biodiversity Recovery In An Environmental Sample
Abstract

A kit may include a reaction receptacle, for example a well of a multi-well plate, for receiving an environmental sample. A kit may include a plurality of primer sets complementary to a taxonomic barcode region of a genome. Each primer set may be disposed in a reaction receptacle. Each primer set may include a first sequence including a sample tag sequence used to identify each environmental sample. Each primer set may include a plurality of primers including one or more forward primers and one or more reverse primers. The plurality of primers does not form or include obligate 1:1 pairs of forward and reverse primers. The plurality of primers may include a taxonomically informative sequence that is complementary to a region of DNA of a taxonomic group. The taxonomically informative sequence may be used to identify whether the taxonomic group is present in one or more environmental samples.

Full Text

What is claimed is:

A kit may include a reaction receptacle, for example a well of a multi-well plate, for receiving an environmental sample. A kit may include a plurality of primer sets complementary to a taxonomic barcode region of a genome. Each primer set may be disposed in a reaction receptacle. Each primer set may include a first sequence including a sample tag sequence used to identify each environmental sample. Each primer set may include a plurality of primers including one or more forward primers and one or more reverse primers. The plurality of primers does not form or include obligate 1:1 pairs of forward and reverse primers. The plurality of primers may include a taxonomically informative sequence that is complementary to a region of DNA of a taxonomic group. The taxonomically informative sequence may be used to identify whether the taxonomic group is present in one or more environmental samples.
Timeline
Filed
04/28/2026
Published
08/20/2026
Granted
Not Available
IPC Codes(2)
C12Q 1/6895:for plants, fungi or algae
B01L 3/00:Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers