A display substrate and a manufacturing method therefor, and a display apparatus. The display substrate includes a plurality of first electrodes (31) and a plurality of pixel definition structures (32) disposed on a base substrate (10). The pixel definition structures (32) are disposed between adjacent first electrodes (31) and forming pixel openings (35) exposing the first electrodes (31). A pixel definition structure (32) includes at least a first insulating layer (101), a second insulating layer (102), a third insulating layer (103), and a fourth insulating layer (104). A surface of the first insulating layer away from the base substrate is provided with at least two first insulating recesses recessed in a direction toward the base substrate. In a direction parallel to the base substrate, a first insulating recess is located between a pixel opening and the third insulating layer. The second insulating layer includes at least a first sub-portion.