The present disclosure discloses a highly efficient and stable method for cis-isomerization of lycopene, where a bimetallic metal-organic framework material is used as a catalyst to catalyze lycopene to undergo cis-isomerization. In the method, confined water molecules may be further added to the reaction system as a catalytic medium to achieve highly efficient catalysis for isomerization of lycopene. The catalyst attacks and activates the conjugated double bonds of all-trans lycopene in an electron-rich environment, thereby enabling rotation to form the corresponding configuration. The confined water molecules serve as an electron transfer medium, accelerating the ability of trans-lycopene to lose electrons to form a lycopene radical intermediate during isomerization, thereby further improving the yield of cis-isomerization of lycopene.