Disclosed herein is a microbiocidal composition, including active ingredients Bacillus velezensis TCS001, boscalid, and Paenibacillus polymyxa HY96-2, where a mass ratio of Bacillus velezensis TCS001 to boscalid is 100:1 to 1:100, and a mass ratio of Bacillus velezensis TCS001 to Paenibacillus polymyxa HY96-2 is 50:1 to 1:50. The microbiocidal composition of this disclosure provides a novel alternative agent for controlling strawberry gray mold, which not only solves the problem of low control efficacy when Bacillus velezensis TCS001 is used alone, but also fully utilizes the disease resistance and immune function of Bacillus velezensis TCS001. Furthermore, through mixed use with boscalid, the development of resistance to boscalid can be delayed or reduced, producing an unexpected synergistic effect. The microbiocidal composition of this disclosure further provides a novel alternative agent for controlling strawberry anthracnose, and the combination of Bacillus velezensis TCS001 and Paenibacillus polymyxa HY96-2 has a synergistic effect in controlling strawberry anthracnose.