The present disclosure is directed to an aerosol delivery device and a holder for use with a removable substrate cartridge. In one implementation, the holder includes a main body defining a proximal end and a distal end, the main body further providing an aerosol passageway that extends through at least a portion of the main body, and a heat sink portion. At least part of the receiving chamber is located in the heat sink portion, and the heat sink portion is configured to dissipate heat from a substrate cartridge. Some implementations further include a cartridge retention assembly and a sliding assembly. The sliding assembly is configured to slide relative to the main body to and from at least a loading position, wherein the cartridge retention assembly is configured to receive a substrate cartridge, and a use position, wherein the cartridge retention assembly is configured to retain a substrate cartridge.