This disclosure concerns methods and compositions for identifying canola plants that have a low fiber content trait. Some embodiments concern a chromosomal interval and a quantitative trait locus associated with low fiber content in canola plants or germplasm. Some embodiments concern molecular markers to identify, select, and/or construct low fiber content canola plants and germplasm, or to identify and counter-select plants with relatively higher fiber content. This disclosure also concerns canola plants comprising a low fiber content trait that are generated by methods utilizing at least one marker described herein.