The present disclosure relates to the field of biotechnology, and in particular to method for modifying a high-yield L-lysine-producing strain engineering and application thereof. The present disclosure provides a recombinant strain obtained by knocking out the cbrB gene and/or the cbrC gene in the genome of E. coli. Fermentation of the recombinant strain can increase the yield and conversion rate of L-lysine and reduce the mortality rate of the strain during the fermentation process. The present disclosure has significant application value. Through experimental studies, it was found that these two genes have a synergistic effect on E. coli. L-lysine hydrochloride imposes osmotic stress resistance on the strains, and silencing the related gene proteins can alter the membrane lipid composition (increasing a proportion of unsaturated fatty acids), maintain membrane fluidity, improve tolerance to lysine hydrochloride, and reduce the mortality rate in the late stage of fermentation.