Disclosed herein are methods for sequencing, comprising, providing a sample, wherein the sample comprises a plurality of nucleic acid (NA) molecules, isolating the plurality of NA molecules from the sample, amplifying the plurality of NA molecules, subjecting the plurality of NA molecules to one or more amplification reactions to generate a plurality of cDNA molecules, and sequencing the plurality of cDNA molecules or derivatives thereof. Also disclosed herein are systems, comprising a processor and a non-transitory computer readable storage medium encoded with a computer program that causes the processor to provide a sample, wherein the sample comprises a plurality of NA molecules, isolating the plurality of NA molecules from the sample, amplifying the plurality of NA molecules, subjecting the plurality of nucleic acid molecules to one or more amplification reactions to generate a plurality of cDNA molecules, and sequencing the plurality of cDNA molecules or derivatives thereof.