Abstract
Thermophilic purified Type A DNA-dependent DNA polymerases efficiently catalyze reverse transcription and RNA-dependent DNA pyrophosphorolysis using ribonucleic acid (RNA) templates, virtually also being reverse transcriptases. At optimal temperatures of 60° C. to 62° C., the thermophilic reverse transcriptases are particularly efficient for reverse transcription in RT-PCR and RT-PAP amplifications.
Full Text
What is claimed is:
Thermophilic purified Type A DNA-dependent DNA polymerases efficiently catalyze reverse transcription and RNA-dependent DNA pyrophosphorolysis using ribonucleic acid (RNA) templates, virtually also being reverse transcriptases. At optimal temperatures of 60° C. to 62° C., the thermophilic reverse transcriptases are particularly efficient for reverse transcription in RT-PCR and RT-PAP amplifications.
Timeline
Filed
05/17/2026Published
09/03/2026Granted
Not AvailableIPC Codes(2)
C12P 19/34:Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 9/12:transferring phosphorus containing groups, e.g. kinases (2.7)