Abstract
A system for multiplex expression of proteins in eukaryotic cells is provided wherein cells simultaneously expressing each of the proteins of the system in a cell can be selected and/or visualized. The system comprises a plurality of lentiviral based vectors that each allow for the expression of the protein of interest, and a fluorescent protein linked to a fusion peptide comprising a proteolytic cleavage site that itself is linked to an antibiotic resistance protein. Each of the vectors of the system has a separate and unique fluorescent protein encoding gene and/or a separate and unique antibiotic resistance gene.
Full Text
What is claimed is:
A system for multiplex expression of proteins in eukaryotic cells is provided wherein cells simultaneously expressing each of the proteins of the system in a cell can be selected and/or visualized. The system comprises a plurality of lentiviral based vectors that each allow for the expression of the protein of interest, and a fluorescent protein linked to a fusion peptide comprising a proteolytic cleavage site that itself is linked to an antibiotic resistance protein. Each of the vectors of the system has a separate and unique fluorescent protein encoding gene and/or a separate and unique antibiotic resistance gene.
Timeline
Filed
05/28/2026Published
09/17/2026Granted
Not AvailableIPC Codes(3)
C12N 15/10:Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H 21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P 19/34)
C12N 15/65:using markers (enzymes used as markers C12N 15/52)
C12N 15/86:Viral vectors