Disclosed herein are methods for determining intracranial pressure (ICP) of a subject non-invasively. One example method may include applying at least one selected controlled force to an eye of the subject, imaging retinal vascular pulsation of the eye of the subject over at least one cardiac cycle at the at least one selected controlled force, processing the retinal vascular pulsation images to produce retinal vascular pulsation amplitude data as a time-varying signal, decomposing the time-varying signal into at least two frequency components representing the time-varying signal, determining intracranial pressure from a data basis which includes at least information associated with the frequency components, and wherein the retinal vascular pulsation data includes at least retinal arterial pulsation data. Also disclosed are systems and computer readable media for determining intracranial pressure non-invasively.