The present invention provides a composition for sleep and mood support, which comprises composition for sleep and mood support, which consists essentially of Xylaria nigripes extract, Panax notoginseng extract and Cuscuta chinensis extract, as main active components at a relative ratio to provide a synergistic effect in increasing GABA content, and GABA-related enzyme activities, and decreasing β-amyloid protein aggregation. Also provided is the use of the composition for manufacturing a formulation for treating insomnia, depression and/or various neurological conditions.