/Age dependent and exposure duration had a significant effect on the survival of the Jeddah and resistant Cayman. Our results showed that in a single exposure assays, age had no significant effect on mortality in the Cayman strain (χ²=2.76, df=1, P=0.097), but there was significantly increased mortality in the Jeddah strain younger age (χ²=5.46, df=1, P=0.02), but not statistically significant at older age. In the multiple exposure assay, GLiM analysis showed a significant strain, day and strain*day interaction indicating mortality rate is influenced by the strain or day (which also corresponds to age).The Jeddah strain showed generally lower survival, there was a highly significant association of survival with repeated exposures in the Jeddah strain (χ²=43.6, df=1, P=4.1×10⁻¹¹) and the Cayman strain (χ²=12.5, df=1, P=0.0004). Mortality rate correlated statistically and significantly with the number of days of exposure in the Cayman strain (Spearman rank correlation ρ=-0.77, P=0.01), but in the Jeddah strain it was not statistically significant (ρ= -0.42, P=0.23). After repeated insecticide exposure, the AAEL006013 was statistically and significantly over-expressed compared to the control (P=0.03).