/A total of 57,477 film images were collected, which yield ~40 million initial particles. Further 2D and 3D classifications generate two maps at resolutions of 2.92 Å and 3.09 Å. The data and structure statistics are summarized in Extended Data Table 1. Both maps were sufficiently clear to place NTSR1, NTS, GRK2, Gαq, and the bound SBI-553, staurosporine, and GDP·AlF4− ·Mg2+.