/For validation of the simulation results, and in order to assess a potential functional impact of the FcγRIIb-I232T transmembrane polymorphism on binding of IgG, we compared IgG immune complex binding to primary human B cells. In line with previous observations of IgG interaction with human FcγRIIb, binding was most pronounced for IgG3 and IgG1 followed by IgG4 and IgG2. Most importantly, however, we did not observe significant differences in binding with respect to the two allelic variants of FcγRIIb.