/Transient hypoxia reduces respiration by inhibiting mitochondrial Complex IV, decreasing cellular ATP levels, and is expected to increase the rate of glycolysis due to energy stress. Thus, manipulation of transient hypoxia in living animals allows us to directly control dynamics of glycolysis. We observed that under normoxic conditions, HYlight displayed a broad range of ratios in different neurons, consistent with varying glycolytic states (and FBP levels) across cells. In contrast, control HYlight-RA displayed low levels of signal in all cells. Upon exposure to transient hypoxia, we observed an increase in the HYlight ratio within one to two minutes, which was not observed in animals expressing HYlight-RA.