/We have previously established a FSGS-like disease model by treating the transgenic zebrafish strain Cherry with 80 μM MTZ. The objective of this study was to investigate whether Nifurpirinol (NFP) can be used as an alternative substrate for the bacterial NTR to injure specifically podocyte. To analyze the functionality of the filtration barrier, we have taken the edema formation as a first readout. The larvae were classified into different categories dependent on the severity of the edema after the treatment (Fig. 1A). Our grading describes four categories starting from 1 (no edema) to 4 (severe edema with bend body axis) shown in Figure 1C. Titration experiments revealed that NFP induced edema formation in 3% of the larvae (at 5 dpf) at concentrations as low as 25 nM. The amount of edemas increased up to 48.5% at 8 dpf. By using 50 nM NFP, 6.1% of the larvae developed edema at 5 dpf, rising to 66.7% at 8 dpf. A further increase of the concentration of NFP up to 100 nM induced in 75.9% of the larvae edema at 5 dpf and 89.7% at 8 dpf, respectively. We used 250 nM NFP as the highest concentration and found that 90.6% of larvae developed edema at 5 dpf and 93.8% at 8 dpf, showing that we have still the dynamic range of the prodrug.