/Combined data from nanopore BARSEQ and Native barcoding, both sequenced on R10.4.1 and assembled with Flye, gave highest scores in CheckM genome completeness for all three strains (99.5%) compared to all other sequencing strategies. In general, high completeness CheckM scores (> 95%) could be achieved with all sequencing strategies either with Flye, HGAP3, and Spade for ONT, PacBio, and Illumina, respectively, or through hybrid assembly utilizing Unicycler (Figure 3 and supplementary Figure S3).