/To identify genes that may be regulated by Pnt, we performed ChIP-seq using late larval eye-antennal discs derived from pnt-GFP-FPTB; pntΔ⁸⁸/pnt2 animals. The pnt-GFP-FPTB is a transgenic genomic clone encoding a Pnt protein with GFP and FLAG tags on its C-terminus. We performed ChIP-seq with anti-GFP or anti-FLAG antibodies with two or three biological replicates, respectively. Each sample was sequenced to a depth of 40 million reads. The unique reads were then mapped to the Drosophila melanogaster genome release 6 (dm6), resulting in an average of 6,362 (8,787 and 3937) and 6,268 (6902, 4903 and 6998) regions (peaks) enriched by anti-GFP or anti-FLAG, respectively.