/We also evaluated our assemblies using BUSCO v.4.1.1 with the brassicales dataset (brassicales_odb10). Compared to the reference assembly, we found that our single flow cell nanopore assemblies performed less well, with between 20% and 40% of genes for the dataset missing, and about 10% fragmented (Table 3). Notably, the assembly by Pomoxis, which was polished with Medaka, recovered significantly more BUSCO genes than all the other assemblies.